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Books : Molecular Cloning: A Laboratory Manual (3-Volume Set)

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by: Joe Sambrook

Books : Molecular Cloning: A Laboratory Manual (3-Volume Set)

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Binding: Paperback
Dewey Decimal Number: 572.8
EAN: 9780879695774
ISBN: 0879695773
Label: Cold Spring Harbor Laboratory Press
Manufacturer: Cold Spring Harbor Laboratory Press
Number Of Items: 3
Number Of Pages: 999
Publication Date: January 15, 2001
Publisher: Cold Spring Harbor Laboratory Press
Studio: Cold Spring Harbor Laboratory Press
Sales Rank: 111167




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Editorial Review:

Product Description:
The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity.

In this new edition, authors Joe Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology.

Handsomely redesigned and presented in new bindings of proven durability, this three-volume work is essential for everyone using today’s biomolecular techniques.

The opening chapters describe essential techniques, some well-established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small.

These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing.

The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein-protein interactions.

The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information.

As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved.



Customer Reviews
Average Rating:  out of 5 stars

Rating: 3 out of 5 stars - Molecular Cloning Manual
The books arrived in good condition, but it took a long time to arrive here.



Rating: 5 out of 5 stars - Molecular cloning: a lab Manual
This manual is high quality for the study of biotechnology. The authors collected lot of protocols and provided more detail of principle than previous two editions. The third edition involved three volumes. The manual is very useful for lab researcher.



Rating: 5 out of 5 stars - Molecular Cloning: A Laboratory Manual (3-Volume Set)
This revised version of the Standard Lab Handbook has been improved, completed with new techniques and search is facilitated by the indices added at the end of each volume.



Rating: 3 out of 5 stars - a formerly essential classic
For many years the previous edition of this set was an essential reference in molecular biology labs. At present however, there are too many good protocol books out there to really make this argument. The book is pretty strong in explaining theory, and answering the question of why certain procedures are either necessary, useful, or worthless, however it is not as practical as many other books, such as Short Protocols. Still a good reference overall, but no longer stands alone, and I recommend ... Read More



Rating: 5 out of 5 stars - the BIBLE of every biologist
So few and so much to say about this bible of Biology at the bench...
You'll really find everything you want in it, including the composition of all the buffers and solutions, the new protocols for high-tech biology (FLIM-FRET), some paragraphs about bioinformatics and more.Incredibly precise, this book is consequently a big book (3 huge volumes), so better know exactly wath you're looking for before opening it!
The must have of every lab!

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